姓名：蔡圣
职称/职务：副教授，博士生导师，浙大二院兼聘副教授，浙江大学临床药学研究中心副主任，太阳集团官网太阳成集团tyc151cc副院长
专业：药物分析学
研究方向：1.药物分析新方法新技术研究。包括功能纳米材料、核酸适配体技术在核酸、蛋白及药物检测中的应用；核酸扩增方法在药物和药物效应分子（核酸、蛋白等）检测和成像中的应用；2.新型肿瘤标志（包括miRNA、DNA甲基化等）的筛选和开发，在临床早期诊断、预后预测和精准治疗中的应用。3.蛋白多肽类、核酸类药物的体内药物代谢动力学研究,药物研发。
邮箱：caisheng@xju.edu.cn
办公室：太阳集团官网博达校区药物研究所208A室
个人经历
学习经历：
2008.9–2013.6 复旦大学太阳成集团tyc151cc，药剂学，博士研究生
2003.9–2007.6浙江大学太阳成集团tyc151cc，药学，本科
工作经历：
2019 -至今浙江大学太阳成集团tyc151cc，药物代谢和分析，副教授
2020- 2021国家科技部重大专项司，业务一处，借调干部
2015- 2016美国德州大学奥斯汀分校，系统生物与合成生物，访问学者
2013-2019浙江大学太阳成集团tyc151cc，药物代谢和分析，讲师
主讲课程
本科生课程：
《药物分析学》
《生物化学与分子生物学》
《药物分析学实验》
研究生课程：
《高等药物分析》
《分析药理学》
《实用药物分析》
主持科研项目
1.国家自然科学基金：基于适配体识别和硫代修饰环介导扩增技术联用的DNA甲基化检测方法研究及其在临床精准用药中的应用（82373828，2024.1- 2027.12，49万，项目负责人）
2.中国人民解放军军委科技委 生物安全专项:模拟人呼吸模拟装置设计与研制（2022G32032，2023.11- 2025.12，200万，课题负责人）
3.国家重大专项：基于生物标志物的农药毒性评估替代方法研究（2023170406，2023.9-2026.12，110万，子课题负责人）
4.国家自然科学基金：基于配位结合的铂离子化学发光适配体检测新技术（21405136，2015.1- 2017.12，25万，项目负责人）
5.国家重点研发计划：基于CRISPR-Cas技术的生物毒素高通量快检技术研究及方法建立（2023YFD1400902, 2023.12-2027.12，40万，子课题负责人）
6.浙江省自然科学基金：基于环介导扩增技术和发夹构象转化的铂类药物检测新方法研究（LY18H300003，2018.1-2021.12，8万，项目负责人）
7.浙江省自然科学基金：基于适配体构象转化和信号放大的MicroRNA化学发光检测新技术（LQ14H300002，2014.1-2016.12，5万，项目负责人）
8.浙江省科技厅《小分子药物研发关键技术创新团队》：基于构象转化的siRNA化学发光适配体检测新技术研究（2011R50014，2012.7-2015.7，10万，子课题负责人）
社会工作
中国生物医药技术协会药物分析技术专委会委员、青委会副主任委员，
中国药理学会表观遗传药理学专委会委员，
中国药理学会分析药理学专委会委员，
浙江省药学会药物分析专业委员会青年委员会副主任委员
Separations杂志客座编辑，Journal of Pharmaceutical Analysis、《沈阳药科大学学报》、《中国现代应用药学》杂志青年编委。
代表性研究成果
2024年
1.Zhaleh Asadi Fakhr, Xinlan Zhu, Hechen Wang, Ruiyang Ma, Ziwei Lin, Xudan Shen, Jiatong Liu, Su Zeng*,Sheng Cai*.Embarking on a journey through Micro-RNA and Circular-RNAdetection methods.Trends in Analytical Chemistry,2024,181,118035.
2.Xudan Shen, Ziwei Lin, Xianfeng Jiang, Xinlan Zhu, Su Zeng,Sheng Cai*and Hui Liu*.Dumbbell Probe Initiated Multi-Rolling Circle Amplification Assisted CRISPR/Cas12a for Highly Sensitive Detection of Clinical microRNA.Biosensors & Bioelectronics,2024, 264, 116676.
3.Hechen Wang, Xudan Shen, Jiatong Liu,Xinlan Zhu, Su Zeng,Sheng Cai*.Enhancing HepG2 cell apoptosis with a combined nanoparticledelivery of miR-128-3p agomir and Oroxin B: A novel drug deliveryapproach based on PI3K-AKT and VEGF pathway crosstalk.Asian Journal of Pharmaceutical Sciences,2024, DOI10.1016,100909.
4.Hechen Wang, Minzhe Shen, Xudan Shen, Jiatong Liu, Wenwen Huang, Xianfeng Jiang, Hui Liu, Su Zeng, Kewang Nan*,Sheng Cai*.An enzyme-free sensing platform for miRNA detection and in situ imaging in clinical samples based on DNAzyme cleavage-triggered catalytic hairpin assembly.Biosensors & Bioelectronics,2024, 256, 116279.
5.Hecheng Wang, Jiatong Liu, Yanyan Fang, Xudan Shen, Hui Liu, Lushan Yu, Su Zeng,Sheng Cai*,Jingjing Zhou*, Zheyong Li*.Design and analysis of self-priming extension DNA hairpin probe for miRNAdetection based on a unified dynamic programming framework.Analytica Chimica Acta, 2024,1303,342530.
6.PeixiZhu,LuxiZhou,YuxiuLin,YixiWang,YuHanandSheng Cai*.A magnetic beads-based ligand fishing method Coupled with UHPLC-QTOF MS for screening and identification of α-glucosidase inhibitors from Houttuynia cordata Thunb.Talanta,2024, 270, 125583.
2023年
7.Xudan Shen, Mingcheng Xu, Hechen Wang, Hua Wang, Minzhe Shen, Jadera Talap, Haihong Hu, Su Zeng*, Shunxiang Gao*,Sheng Cai*.Site-specific detection of circulating tumor DNA methylation in biological samples utilizing phosphorothioated primer-based loop-mediated isothermal amplification.Biosensors & Bioelectronics,2023, 237, 115550.
8.Jadera Talap, Abdu Ahmed Abdullah AL-maskri, Minzhe Shen, Hui Liu, Xianfeng Jiang, Guizhou Xiao, Lushan Yu, Su Zeng*, Cheulhee Jung*,Sheng Cai*.Ultrasensitive detection of serum miRNA biomarkers related to papillary thyroid cancer using ligation-initiated phosphorothioated primer-based loop-mediated isothermal amplification.Sensors and Actuators: B. Chemical,2023, 374, 132785.
9.Yu Han, Haihong Hu, Lushan Yu, Su Zeng, Jun Zhe Min*,Sheng Cai*.A duplex-specific nuclease (DSN) and catalytic hairpin assembly (CHA)-mediated dual amplification method for miR-146b detection.Analyst,2023, 148, 556-561.
2022年
10.Xianfeng Jiang, Xudan Shen, Jadera Talap, Dan Yang, Su Zeng, Hui Liu*,Sheng Cai*.Phosphorothioated and phosphate-terminal dumbbell (PP-TD) probe-based rapid detection of polynucleotide kinase activity.Analyst,2022, 147, 4986-4990.
11.Jadera Talap, Minzhe Shen, Lushan Yu, Su Zeng*,Sheng Cai*.RT-LAMP assay combining multi-fluorescent probes for SARS-CoV-2 RNA detection and variant differentiation.Talanta,2022, 248, 123644.
12.Abdu Ahmed Abdullah AL-maskri, Guangbo Jin, Yang Li, Jadera Talap, Marwan Almoiliqy, Chowdhury Apu, Su Zeng, Ying Zhou*,Sheng Cai*.A self-assembly amplification strategy for ultra-sensitive detection of microRNA based on phosphorothioated probes.Talanta,2022, 249, 123618.
13.Jiatong Liu, Minzhe Shen, Jadera Talap, Xudan Shen, Zihan Song, Haihong Hu, Su Zeng*,Sheng Cai*. A sensitive isothermal fluorescence biosensor for microRNA detection coupling primer exchange reaction with catalytic hairpin assembly.Sensors & Diagnostics, 2022, 1, 1063-1068.
14.Xianfeng Jiang, Jiahui Wang, Xudan Shen, Jadera Talap, Yang Yang, Dan Yang, Guizhou Xiao*andSheng Cai*.A Novel Helper qPCR Assay for the Detection of miRNA Using Target/Helper Template for Primer Formation.International Journal of Analytical Chemistry, 2022, 2022, 6918054.
15.Zihan Song, Yun Zhou, Minzhe Shen, Dong Zhao, Haihong Hu, Su Zeng, Lianli Sun* andSheng Cai*.MUC1 detection and in situ imaging method based on aptamer conformational switch and hybridization chain reaction.Talanta,2022, 239, 123129.
2021年
16.Zheng Cao, Xianfeng Jiang, Guizhou Xiao, Mingcheng Xu, Hui Liu* andSheng Cai*.Rolling Circle and Loop Mediated Isothermal Amplification Strategy for Ultrasensitive miRNA Detection.Separations,2021, 8, 166.
17.Jadera Talap, Jing Zhao, Minzhe Shen, Zihan Song, Hui Zhou, Yu Kang, Lianli Sun, Lushan Yu, Su Zeng*,Sheng Cai*.Recent advances in therapeutic nucleic acids and their analytical methods.Journal of Pharmaceutical and Biomedical Analysis, 2021, 206, 114368.
2020年
18.Abdu Ahmed Abdullah AL-maskri, Jiawei Ye, Jadera Talap, Haihong Hu, Lianli Sun, Lushan Yu,Sheng Cai*, Su Zeng*.Reverse transcription-based loop-mediated isothermal amplification strategy for real-time miRNA detection with phosphorothioated probes.Analytica Chimica Acta,2020, 1126,1-6.
19.Minzhe Shen,Ying Zhou, Jiawei Ye, Abdu Ahmed Abdullah AL-maskri, Yu Kang, Su Zeng*,Sheng Cai*. Recent advances and perspectives of nucleic acid detection for coronavirus.Journal of Pharmaceutical Analysis, 2020, 10,97-101.
2019年
20.Sheng Cai,Dan Yang, Xueke Tian, Jiawei Ye, Mingcheng Xu, Abdu Ahmed Abdullah AL-maskri, Cheulhee Jung*, Su Zeng*.A Novel Helper qPCR System for Platinum Detection via Pt-DNA Coordination.Analytica Chimica Acta,2019,1050, 154-160.
21.Shunxiang Gao, Wei Hu, Xin Zheng*,Sheng Cai*, Jihong Wu*. Functionalized aptamer with an antiparallel G-quadruplex: Structural remodeling, recognition mechanism, and diagnostic applications targeting CTGF.Biosensors & Bioelectronics,2019,142, 111475.
22.Mingcheng Xu,Jiawei Ye, Dan Yang,Abdu Ahmed Abdullah AL-maskri,Haihong Hu,Cheulhee Jung,Sheng Cai*,Su Zeng*.Ultrasensitive detection of miRNA via one-step rolling circle-quantitative PCR (RC-qPCR).Analytica Chimica Acta, 2019,1077,208-215.
23.Jiawei Ye,Xueke Tian, Mingcheng Xu,Sheng Cai*,Su Zeng*.Research advances in the detection of miRNA.Journal of Pharmaceutical Analysis, 2019, 9,217-226.
24.Sheng Cai,YingZhou,Jiawei Ye,Ruizhe Chen,Lianli Sun,Jianzhong Lu,Cheulhee Jung*, Su Zeng*.A chemiluminescence resonance energy transfer strategy and its application for detection of platinum ions and cisplatin.Microchimica Acta, 2019,186, 463.
25.Sheng Cai, Nana Shao, Yuanyuan Chen, Anbang Li, Jie Pan, Huajian Zhu, Hongbin Zou, Su Zeng, Lianli Sun*, Jinhao Zhao*. Enantioselective Reduction of α, β-Unsaturated Ketones and Aryl Ketones by Perakine Reductase.Organic letters, 2019,21,4411-4414.
26.Sheng Cai,Jiawei Ye,Abdu Ahmed Abdullah AL-maskri,Lianli Sun*,Su Zeng*.A conformational switch‐based aptasensor for the chemiluminescence detection of microRNA.Luminescence, 2019, 34, 823–829.
2018年
27.Sheng Cai,Cheulhee Jung, Sanchita Bhadra, Andrew D Ellington*. Phosphorothioated Primers Lead to Loop-Mediated Isothermal Amplification at Low Temperatures.Analytical Chemistry,2018,90, 8290-8294.
28.YuJiangSherry, Timothy E Riedel, Jessica A Popoola, Barrett R Morrow,Sheng Cai,Andrew D. Ellington, Sanchita Bhadra*. Portable platform for rapid in-field identification of human fecal pollution in water.Water Research, 2018, 131, 186-195.
2015年之前
29.Sheng Cai, Xueke Tian, Lianli Sun, Haihong Hu, Shirui Zheng, Huidi Jiang, Lushan Yu, Su Zeng*.Platinum (II)-Oligonucleotide Coordination Based Aptasensor forSimple and Selective Detection of Platinum Compounds.Analytical Chemistry,2015,87,10542-10546.
30.Sheng Cai,Zhijuan Cao,Choiwan Lau and Jianzhong Lu*. Label-free technology for the amplified detectionof microRNA based on the allosteric hairpin DNAswitch and hybridization chain reaction,Analyst,2014, 139, 6022–6027.
31.Sheng Cai, Yanhua Sun, Choiwan Lau, Jianzhong Lu*.Sensitive chemiluminescence aptasensor based on exonuclease-assisted recycling amplification.Analytica Chimica Acta,2013,761, 137-142.
32.Sheng Cai, Choiwan Lau and Jianzhong Lu*.“Turn-On” Chemiluminescence Sensor for the Highly Selective and Ultrasensitive Detection of Hg2+Ions Based on Interstrand Cooperative Coordination and Catalytic Formation of Gold Nanoparticles.Analytical Chemistry,2011, 83,9702-9708.
33.Sheng Cai, Choiwan Lau and Jianzhong Lu*. Turn-on Aptameric System for Simple and Selective Detection of Protein via Base Stacking-dependent DNA Hybridization Event.Analytical Chemistry,2011,83, 5844-5850.
34.Sheng Cai,Liang Xin, Choiwan Lau, Jianzhong Lu*, Xueji Zhang. Ultrasensitive and selective DNA detection by hydroxylamine assisted gold nanoparticle amplification.Chemical Communications,2011, 47, 6120-6122.
35.Yanhua Sun,ShengCai, Zhijuan Cao, Choiwan Lau and Jianzhong Lu*, Aptameric system for the highly selective and ultrasensitive detection of protein in human serum based on non-stripping gold nanoparticles.Analyst,2011, 136, 4144-4151.
36.Sheng Cai, Choiwan Lau and Jianzhong Lu*, Sequence-Specific Detection of Short-Length DNA via Template-Dependent Surface-Hybridization Events.Analytical Chemistry,2010, 82, 7178-7184.
37.Sheng Cai,Liang Xin, Choiwan Lau and Jianzhong Lu*. Highly sensitive non-stripping gold nanoparticles-based chemiluminescent detection of DNA hybridization coupled to magnetic beads.Analyst,2010, 135, 615-620.
38.AipingFan,Sheng Cai, Zhijuan Cao, Choiwan Lau and Jianzhong Lu*.Hydroxylamine-amplified gold nanoparticles for the homogeneous detection of sequence-specific DNA.Analyst,2010, 135, 1400-1405.
授权专利
1.基于硫代修饰环介导等温扩增法的miRNA检测试剂盒。发明人：蔡圣，加德拉·塔拉甫，沈敏哲，余露山，曾苏。专利号：202011031962.5，授权时间2021.10.19。
2.一种单个位点DNA甲基化检测试剂盒。发明人：蔡圣，徐鸣成，余露山，曾苏。专利号：201911240425.9，授权时间2021.8.3。
3.冠状病毒RNA双基因同时检测及突变毒株识别的检测试剂盒。发明人：蔡圣，加德拉·塔拉甫，曾苏。专利号：202111473455.1，授权时间2022.6.28。
4.METHODS AND DEVICES RELATED TO AMPLIFYING NUCLEIC ACID AT A VARIETY OF TEMPERATURES.Andrew Ellington, Cheulhee Jung, Sheng Cai, Sanchita Bhadra, John N. Milligan, Daniel Garry, Raghav Shroff.专利号US 11299776,授权时间2022.4.12。
